Insertion junction: LMJ.RY0402.158906_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.158906
Chromosome:chromosome 17
Location:5559392
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g737525 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGATAAATGGAAACTGCCTGAAACTGTTG
Internal bar code:AGGCGACGGGAGATACTCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2249
LEAP-Seq percent confirming:89.9319
LEAP-Seq n confirming:72316
LEAP-Seq n nonconfirming:8096
LEAP-Seq n unique pos:142

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTGCGTATTTCGAACACC
Suggested primer 2:COULD_NOT_FIND_PRIMER