Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.158916 |
Chromosome: | chromosome_9 |
Location: | 276706 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g392867 | FMG1-B | Flagella membrane glycoprotein, major form | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGTCTTGTGCACTTGGGATGCTAGCGCGGA |
Internal bar code: | AAACTAAACGCAGCCCCTAGAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 762 |
LEAP-Seq percent confirming: | 97.5999 |
LEAP-Seq n confirming: | 32003 |
LEAP-Seq n nonconfirming: | 787 |
LEAP-Seq n unique pos: | 140 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTGCTCAACGTCACCTACA |
Suggested primer 2: | TCAGCAGGCTACATGCATTC |