| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.158916 |
| Chromosome: | chromosome 9 |
| Location: | 276706 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392867 | FMG1-B,FMG2,FMG1B | Flagella Membrane Glycoprotein 1B | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCTTGTGCACTTGGGATGCTAGCGCGGA |
| Internal bar code: | AAACTAAACGCAGCCCCTAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 762 |
| LEAP-Seq percent confirming: | 97.5999 |
| LEAP-Seq n confirming: | 32003 |
| LEAP-Seq n nonconfirming: | 787 |
| LEAP-Seq n unique pos: | 140 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCTCAACGTCACCTACA |
| Suggested primer 2: | TCAGCAGGCTACATGCATTC |