| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.158916 |
| Chromosome: | chromosome_9 |
| Location: | 276735 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g392867 | FMG1-B | Flagella membrane glycoprotein, major form | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAAACGCCCTTCTGGGGCTGAAGTCAGTCT |
| Internal bar code: | ACAGCTTTAGCAAGACCATCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 309 |
| LEAP-Seq percent confirming: | 98.2405 |
| LEAP-Seq n confirming: | 335 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCTCAACGTCACCTACA |
| Suggested primer 2: | TCAGCAGGCTACATGCATTC |