| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.158919 |
| Chromosome: | chromosome 3 |
| Location: | 1757525 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g153900 | FMO11 | Flavin-containing monooxygenase; (1 of 1) IPR002938//IPR006076//IPR011032 - FAD-binding domain // FAD dependent oxidoreductase // GroES-like | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGGTGGATTGTACGCGTGAGATATTGA |
| Internal bar code: | ATGGTTGCGTTAACGATTACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 179 |
| LEAP-Seq percent confirming: | 95.8333 |
| LEAP-Seq n confirming: | 184 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATGTGGTGTGCGTATGTG |
| Suggested primer 2: | AACGTGTTACGTGCGTGTGT |