| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.158927 |
| Chromosome: | chromosome 17 |
| Location: | 505149 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g699400 | (1 of 6) PTHR10994//PTHR10994:SF72 - RETICULON // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACGGCTGCTGCACTGCACGCCTTCCAC |
| Internal bar code: | CGAGCCGTTTCCGCCGCGCACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 801 |
| LEAP-Seq percent confirming: | 99.4358 |
| LEAP-Seq n confirming: | 6873 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGCTTCTTCGTCTGGAGC |
| Suggested primer 2: | CTGGTGAGGGCATTGGTAGT |