| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.158949 |
| Chromosome: | chromosome 2 |
| Location: | 2393233 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g091200 | (1 of 1) 1.13.11.5 - Homogentisate 1,2-dioxygenase / Homogentisicase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGAGCTGGCTCCGCCGACTGGGATGGCG |
| Internal bar code: | TGTCCTTTTTACTATGGCAACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 321 |
| LEAP-Seq percent confirming: | 99.5829 |
| LEAP-Seq n confirming: | 955 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGTCTCGTTTGGGAAGTT |
| Suggested primer 2: | TCTCATGTTGGAGTTCTGCG |