| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.158949 |
| Chromosome: | chromosome 3 |
| Location: | 299993 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144607 | (1 of 79) IPR011992 - EF-hand domain pair | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGGGGCCCTCGGATGCGCCCAACGGCCC |
| Internal bar code: | GATCACGCGTCCGGAATGCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 168 |
| LEAP-Seq percent confirming: | 62.8829 |
| LEAP-Seq n confirming: | 349 |
| LEAP-Seq n nonconfirming: | 206 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCGAGACGCATTCTCCTT |
| Suggested primer 2: | ACTACCGAGGTGGGGAGGTA |