| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.158949 |
| Chromosome: | chromosome 4 |
| Location: | 3658364 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g228800 | (1 of 19) K08850 - aurora kinase, other [EC:2.7.11.1] (AURKX) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCGTAACCGGTAACCACCGGGACAGGCG |
| Internal bar code: | CGTTCCGGTTGTTAGTCTTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 906 |
| LEAP-Seq percent confirming: | 98.0574 |
| LEAP-Seq n confirming: | 7067 |
| LEAP-Seq n nonconfirming: | 140 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGACATCGAGAGGACGTGA |
| Suggested primer 2: | AGGCATAAACGCGCAGTAGT |