| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.158951 |
| Chromosome: | chromosome 10 |
| Location: | 5792503 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g461400 | (1 of 1) PTHR22957:SF212 - [PROTEIN ADP-RIBOSYLARGININE] HYDROLASE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGACCGGGCAATTGCAAACGAAACCCGT |
| Internal bar code: | GTGGCAACCGCCTGGAGGATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 795 |
| LEAP-Seq percent confirming: | 99.8577 |
| LEAP-Seq n confirming: | 2106 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGGCGCAATCTCAACACA |
| Suggested primer 2: | TAGGAGCTCCGAGATGGAGA |