| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.158951 |
| Chromosome: | chromosome 12 |
| Location: | 7633548 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAATCCAGATACATGGCGTCATCTCTTGG |
| Internal bar code: | CTCAGGCGGCGGGGATCTTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 230 |
| LEAP-Seq percent confirming: | 73.288 |
| LEAP-Seq n confirming: | 1616 |
| LEAP-Seq n nonconfirming: | 589 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTTACTCACTAGAGCGGG |
| Suggested primer 2: | TGTCTGGTTAGCGACCTGTG |