| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.158954 |
| Chromosome: | chromosome_3 |
| Location: | 2917763 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g163000 | POC5 | Centriole proteome protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAACGTGTTCCGCGGCTGGTTCCGTGTGGC |
| Internal bar code: | CAGAGTAGGGCGCGGGCCAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 904 |
| LEAP-Seq percent confirming: | 99.6688 |
| LEAP-Seq n confirming: | 5116 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAAGGCGGTGCTAGATCA |
| Suggested primer 2: | GCCTGCACAGCATTAGAACA |