Insertion junction: LMJ.RY0402.158954_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.158954
Chromosome:chromosome_3
Location:2917763
Confidence (%):58
Locus systematic id Locus common name Defline Orientation Feature
Cre03.g163000 POC5 Centriole proteome protein sense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):CAACGTGTTCCGCGGCTGGTTCCGTGTGGC
Internal bar code:CCAGGCGGGGACTTCAGCATGA

Confirmation - LEAP-Seq

LEAP-Seq distance:176
LEAP-Seq percent confirming:59.8456
LEAP-Seq n confirming:155
LEAP-Seq n nonconfirming:104
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AAGAAGGCGGTGCTAGATCA
Suggested primer 2:GCCTGCACAGCATTAGAACA