Insertion junction: LMJ.RY0402.158957_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.158957
Chromosome:chromosome 17
Location:4129366
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g729650 MPA15 Metallophosphoesterase/metallo-dependent phosphatase; (1 of 1) PTHR35769:SF2 - CALCINEURIN-LIKE METALLO-PHOSPHOESTERASE SUPERFAMILY PROTEIN intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACCCTCCCGACATTCGAGGCCGCCGCA
Internal bar code:TTGGGGGGGGATCACGAGCTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:337
LEAP-Seq percent confirming:1.19478
LEAP-Seq n confirming:76
LEAP-Seq n nonconfirming:6285
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:AACACACTCCCTCCCTCCTT
Suggested primer 2:TACCTTTTCTCAGCGCCACT