Insertion junction: LMJ.RY0402.158957_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.158957
Chromosome:chromosome 17
Location:4129376
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g729650 MPA15 Metallophosphoesterase/metallo-dependent phosphatase; (1 of 1) PTHR35769:SF2 - CALCINEURIN-LIKE METALLO-PHOSPHOESTERASE SUPERFAMILY PROTEIN intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCCGATGATGTTAGAAGGTGTGGTGCGGC
Internal bar code:CCCCGATTAGCGTTGACCGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:264
LEAP-Seq percent confirming:99.5902
LEAP-Seq n confirming:6562
LEAP-Seq n nonconfirming:27
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AACACACTCCCTCCCTCCTT
Suggested primer 2:TACCTTTTCTCAGCGCCACT