| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.158963 |
| Chromosome: | chromosome 6 |
| Location: | 2047200 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g264550 | CGL81 | (1 of 4) PF09366 - Protein of unknown function (DUF1997) (DUF1997); Conserved in the Green Lineage | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGACCTCACGTCCTGCGCTTGCAAGCCTTT |
| Internal bar code: | AGCGTCGTAGCGGCTCGTCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 137 |
| LEAP-Seq percent confirming: | 99.8195 |
| LEAP-Seq n confirming: | 553 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGTTTAACACCCCCTTC |
| Suggested primer 2: | ATCGTTTAACGTTTGCAGGG |