| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.158989 |
| Chromosome: | chromosome 12 |
| Location: | 4400201 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g520700 | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTTGGGCATCGATGCAGCTTGAATCTCT |
| Internal bar code: | CCGCGCATGGATAACACAAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 462 |
| LEAP-Seq percent confirming: | 97.7679 |
| LEAP-Seq n confirming: | 219 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGAAGACGGAGACTCAGG |
| Suggested primer 2: | TCTGAAACGGAGCTTCCCTA |