| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.159004 |
| Chromosome: | chromosome_10 |
| Location: | 2997783 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g440950 | FAP192 | Flagellar Associated Protein | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTCACACAAGAACAGATGTGCATCCCAGCC |
| Internal bar code: | TTAATGCTGACCACAGGGGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 449 |
| LEAP-Seq percent confirming: | 99.2608 |
| LEAP-Seq n confirming: | 2417 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCCGTAGGAGTGTACGGT |
| Suggested primer 2: | GCTGCAGGGTCTGTACAAGG |