| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.159013 |
| Chromosome: | chromosome 17 |
| Location: | 6062746 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g741200 | EB1,EBP1 | (1 of 1) K10436 - microtubule-associated protein, RP/EB family (MAPRE); Microtubule plus-end binding protein EB1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCACGGCCCGCTCTTCGTCACGATGGGC |
| Internal bar code: | GTAGCGGGATAGCCGTGAGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 259 |
| LEAP-Seq percent confirming: | 99.8768 |
| LEAP-Seq n confirming: | 811 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGGCACTCTCTACGCCAT |
| Suggested primer 2: | GAGCAGGAGATCCTGTACGC |