| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.159015 |
| Chromosome: | chromosome 12 |
| Location: | 1563284 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486750 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGACCAATTTGACGTACTGCAAGTAGTAC |
| Internal bar code: | GGACAATCCTGCACAGCCCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 508 |
| LEAP-Seq percent confirming: | 99.2823 |
| LEAP-Seq n confirming: | 415 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGCTAGCTCTGCCCATAC |
| Suggested primer 2: | AATAGCATCATAGCCGGGTG |