| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.159030 |
| Chromosome: | chromosome 1 |
| Location: | 3741655 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g024100 | (1 of 19) IPR018392 - LysM domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATAAAAGTGGTGGTCGCAGTAGTTGACCG |
| Internal bar code: | CGGGTGATCGATCCACGAAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 727 |
| LEAP-Seq percent confirming: | 96.7111 |
| LEAP-Seq n confirming: | 4793 |
| LEAP-Seq n nonconfirming: | 163 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGCATGCAAGCTGTAAGT |
| Suggested primer 2: | GCCAAATGTTACCGCAAACT |