| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.159060 |
| Chromosome: | chromosome 3 |
| Location: | 5407146 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g184900 | (1 of 1) IPR027417//IPR029060 - P-loop containing nucleoside triphosphate hydrolase // PIN domain-like | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGGTTAGGTCAGCGACCGGCAGGGGGAA |
| Internal bar code: | GCGGTGAATATAAAGGAGGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 622 |
| LEAP-Seq percent confirming: | 97.2973 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGCGCATAGTGCAAGGAT |
| Suggested primer 2: | TAGAAGTCGGAGTCGTCGGT |