| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.159088 |
| Chromosome: | chromosome 12 |
| Location: | 1953401 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g510450 | RPS28 | Ribosomal protein S28, component of cytosolic 80S ribosome and 4 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAATGAAGGAGTGCTTGGTGAGCTTTTGCG |
| Internal bar code: | CCGGTAAACTGGGGCCGGCTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 830 |
| LEAP-Seq percent confirming: | 99.4118 |
| LEAP-Seq n confirming: | 1183 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCATTCCCTCCTATTGC |
| Suggested primer 2: | TATTCCCAACATCCCCAAAA |