| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.159094 |
| Chromosome: | chromosome 3 |
| Location: | 8108238 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g200800 | CYG61 | (1 of 11) 4.6.1.1//4.6.1.2 - Adenylate cyclase / ATP pyrophosphate-lyase // Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATTATCGACTCGGCCGGGGCGGCCAGTAG |
| Internal bar code: | CTATGTTAAAATTTCCGCTATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 160 |
| LEAP-Seq percent confirming: | 99.6395 |
| LEAP-Seq n confirming: | 1935 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTCCAGTGTACCTTTGCC |
| Suggested primer 2: | CTCGTCATGTGCGCTAGTGT |