| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.159107 |
| Chromosome: | chromosome 17 |
| Location: | 3728336 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g727300 | NFO2 | (1 of 3) 1.3.1.42 - 12-oxophytodienoate reductase; NADH:flavin oxidoreductase/NADH oxidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATCGTGGCGGGGTCCACCCGCGGCCAGTC |
| Internal bar code: | GGTATGATAAGCCGGCCACCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 864 |
| LEAP-Seq percent confirming: | 99.5229 |
| LEAP-Seq n confirming: | 5215 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGGCTACTGCCTCAAGAC |
| Suggested primer 2: | TGTGGACGCATGGACTATGT |