| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.159109 |
| Chromosome: | chromosome 10 |
| Location: | 72487 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g418050 | (1 of 42) IPR000008 - C2 domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGTGCGGCCGGCGACTCCCGTGCGGCCC |
| Internal bar code: | GTGCATGTCATGTCAAAATATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 325 |
| LEAP-Seq percent confirming: | 94.1563 |
| LEAP-Seq n confirming: | 1289 |
| LEAP-Seq n nonconfirming: | 80 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAACAACTTGCGTTGCTA |
| Suggested primer 2: | ACGCCACATCCAGTTCTACC |