Insertion junction: LMJ.RY0402.159142_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.159142
Chromosome:chromosome 7
Location:4731336
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g345200 (1 of 1) 3.6.1.11 - Exopolyphosphatase / Metaphosphatase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTTACTGTCAACGGATTCGCCCTTGTGA
Internal bar code:TTCACGTGTGACGAGCTAGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:842
LEAP-Seq percent confirming:99.7786
LEAP-Seq n confirming:1352
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TGAAGAAGTTGACTGCGTGG
Suggested primer 2:TCGGTGTTGTTAAATTGGCA