| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.159149 |
| Chromosome: | chromosome 1 |
| Location: | 2882088 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g017500 | (1 of 1) K14574 - ribosome maturation protein SDO1 (SDO1, SBDS) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATGTGCGACCCTGTTATCGTTTGGGGTC |
| Internal bar code: | GCAGTAGCGCAGGAATCGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 576 |
| LEAP-Seq percent confirming: | 92.4429 |
| LEAP-Seq n confirming: | 526 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATCATTCAGGTCTTCCGC |
| Suggested primer 2: | GGTAGGGTTTGGGATGGATT |