| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.159197 |
| Chromosome: | chromosome 2 |
| Location: | 1663216 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g085701 | (1 of 37) PF13639 - Ring finger domain (zf-RING_2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTGTATGAGCAGGAGCGTGGGCATTGAA |
| Internal bar code: | CGTCTATTGGGCCCCCAGTACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1104 |
| LEAP-Seq percent confirming: | 99.8081 |
| LEAP-Seq n confirming: | 2600 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACGGGTACGGATAGCAAG |
| Suggested primer 2: | CCGAAGTAGCGTAGGGACTG |