Insertion junction: LMJ.RY0402.159206_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.159206
Chromosome:chromosome 16
Location:1918263
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g656050 (1 of 26) IPR000209 - Peptidase S8/S53 domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGAACAGGGCCGCACTGGCATCCTTAGAC
Internal bar code:GCGTCCCCCTGGGTCACTAGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:190
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GAAAGGGGAGAACAACAGCA
Suggested primer 2:CCGTGTAAGTGGGTTTTGCT