| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.159207 |
| Chromosome: | chromosome 16 |
| Location: | 6973053 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677205 | (1 of 4) PF00207 - Alpha-2-macroglobulin family (A2M) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGACGTTGAGAACTCACAGTACCAGCCT |
| Internal bar code: | GTGTGGCCCACATTGGGGGTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 766 |
| LEAP-Seq percent confirming: | 99.4144 |
| LEAP-Seq n confirming: | 3735 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAAGTGATGCCTGGTTGTA |
| Suggested primer 2: | TTATGGGCATAGTTCCGCTC |