| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.159239 |
| Chromosome: | chromosome 14 |
| Location: | 3622610 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g631250 | (1 of 1) PTHR12832:SF11 - PROTEIN M05D6.2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTCCTGATGAATTACCTACAAACGAGCA |
| Internal bar code: | TGATGAGACTTTACCATTGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 495 |
| LEAP-Seq percent confirming: | 95.7031 |
| LEAP-Seq n confirming: | 1470 |
| LEAP-Seq n nonconfirming: | 66 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCAGTCGCTGATTCCTGA |
| Suggested primer 2: | GCTGGAGACTCGCTGATACC |