| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.159240 |
| Chromosome: | chromosome 10 |
| Location: | 6431616 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g465900 | CDKA1 | Cyclin-dependent kinase; (1 of 4) K02206 - cyclin-dependent kinase 2 (CDK2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCCCTGTATTGTCCTGCGGCTTGGTGT |
| Internal bar code: | TGAAGGGGTTACTCGCAGAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1057 |
| LEAP-Seq percent confirming: | 99.9197 |
| LEAP-Seq n confirming: | 3732 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGATGTGTGGAGTTATGG |
| Suggested primer 2: | GGGCACTTATGGTGTCGTCT |