Insertion junction: LMJ.RY0402.159241_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.159241
Chromosome:chromosome 3
Location:748478
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g146187 AGPR,NAGPR,ARGC1 (1 of 1) 1.2.1.38 - N-acetyl-gamma-glutamyl-phosphate reductase / NAGSA dehydrogenase; N-acetyl-gamma-glutamyl-phosphate reductase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTTCCCGAGCGCTTCAACGCGGAGCTCAC
Internal bar code:AACGGATGAGTTGCGACGCGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:542
LEAP-Seq percent confirming:99.8886
LEAP-Seq n confirming:2691
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:AGAGACTTCCGCTGCATCAT
Suggested primer 2:ATTGACAACCTGGTCAAGGG