Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.159248 |
Chromosome: | chromosome 15 |
Location: | 1283177 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre15.g641662 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTACCCGGGCATCCGGGAAATTTTTGTTT |
Internal bar code: | ACGTCCGTCCAAGCCCCGCGAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 723 |
LEAP-Seq percent confirming: | 99.8499 |
LEAP-Seq n confirming: | 665 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAAGTAAGTTTCCCGGCGTT |
Suggested primer 2: | TATTTACTGCAGGGGGAACG |