Insertion junction: LMJ.RY0402.159251_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.159251
Chromosome:chromosome 5
Location:3335358
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g240600 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTCCTTCCCCGCTGCTCCTCCGTTAGTTC
Internal bar code:AAGAGCAGGGGATTGTGCCGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:221
LEAP-Seq percent confirming:99.7778
LEAP-Seq n confirming:449
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AGCAAAGTGCTTCTTTCCCA
Suggested primer 2:GTCCCGAGTCACGTAGAAGC