Insertion junction: LMJ.RY0402.159254_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.159254
Chromosome:chromosome 16
Location:1664974
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g654600 PHC56 (1 of 71) PF12499 - Pherophorin (DUF3707); Pherophorin-chlamydomonas homolog 56 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAATTCTCGGTCCTCACCAGCTGCGTCACG
Internal bar code:GACACCCAAGTCACCCAAAGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1044
LEAP-Seq percent confirming:90.4926
LEAP-Seq n confirming:790
LEAP-Seq n nonconfirming:83
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:ATGCCTACCTTACCACGCAC
Suggested primer 2:CGAGTACAGCAGCGTGGATA