| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.159267 |
| Chromosome: | chromosome 16 |
| Location: | 3525257 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677382 | (1 of 24) PTHR10641 - MYB-LIKE DNA-BINDING PROTEIN MYB | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACTTCAGAGTGCCAGCTTCGCACTTTTG |
| Internal bar code: | CCTGGGTAGGTGTGGTATGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 596 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 127 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTACAGCTGAAGGGGCAG |
| Suggested primer 2: | CTCGAAATCGCTGAAAGGAC |