| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.159275 |
| Chromosome: | chromosome 2 |
| Location: | 4034862 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g097213 | (1 of 1) PTHR19331//PTHR19331:SF322 - LYSYL OXIDASE-RELATED // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGGCTGCCGGCCGCGTCCTCTGGGATGA |
| Internal bar code: | GTTGCAGTCTAGCTGCCCAACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 387 |
| LEAP-Seq percent confirming: | 97.5251 |
| LEAP-Seq n confirming: | 17851 |
| LEAP-Seq n nonconfirming: | 453 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTGTACGGCATGGTTTTG |
| Suggested primer 2: | TCGCATGTTAGCAGAGAACG |