| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.159280 |
| Chromosome: | chromosome 7 |
| Location: | 6045298 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g355050 | (1 of 1) IPR003610 - Carbohydrate-binding module family 5/12 | intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCCACCCCAACTGCCCCCTAGAGTGAAC |
| Internal bar code: | ACGCGGTATTAGGGGGTCGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 593 |
| LEAP-Seq percent confirming: | 98.8487 |
| LEAP-Seq n confirming: | 601 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCTAATGCAACGGCAGAG |
| Suggested primer 2: | ATACCCACCAGTCTGCGTTC |