| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.159308 |
| Chromosome: | chromosome 9 |
| Location: | 3946289 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392430 | (1 of 1) 1.1.1.62 - 17-beta-estradiol 17-dehydrogenase / Estrogen 17-oxidoreductase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCTCATTACGGATGTGGGATGAAAGGTA |
| Internal bar code: | GGGTGTGGCAGCTCTATATTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 757 |
| LEAP-Seq percent confirming: | 99.8371 |
| LEAP-Seq n confirming: | 4904 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGGCTGATTACACGGAAC |
| Suggested primer 2: | CCCAAAACTGCACAACCTTT |