| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.159325 |
| Chromosome: | chromosome 14 |
| Location: | 2223447 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g622901 | (1 of 21) IPR001611//IPR003591 - Leucine-rich repeat // Leucine-rich repeat, typical subtype | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGACGGCATTCCCTTCTCCCCCCTGAAC |
| Internal bar code: | GGGTCATCATGCAAGTCCGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 619 |
| LEAP-Seq percent confirming: | 96.7227 |
| LEAP-Seq n confirming: | 1151 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTAGCGGAACAACTTGGA |
| Suggested primer 2: | CATGTGTCGCAGCAGGTACT |