Insertion junction: LMJ.RY0402.159330_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.159330
Chromosome:chromosome 2
Location:5259462
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g106350 CGL68,HAP5 (1 of 1) PTHR31446:SF5 - ACID PHOSPHATASE/VANADIUM-DEPENDENT HALOPEROXIDASE-RELATED PROTEIN; Acid phosphatase/vanadium-dependent haloperoxidase related, DUF212 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTGATGTGAACTCGGTAGACTTTCGGGCT
Internal bar code:CAGTTTGGTTAAGGGCGCGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:526
LEAP-Seq percent confirming:99.8742
LEAP-Seq n confirming:794
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GCTCTTACCAGTTGCCTTGC
Suggested primer 2:GCCTTGAGTGGTCTAGCAGG