| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.159340 |
| Chromosome: | chromosome 3 |
| Location: | 6598214 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g196350 | (1 of 3) K09858 - SEC-C motif domain protein (K09858) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTTTAGCAACCGCGCGTACACGCCCGAT |
| Internal bar code: | CCGGCATCACTATGCCCAGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 357 |
| LEAP-Seq percent confirming: | 99.7245 |
| LEAP-Seq n confirming: | 9772 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGATGAGGCGACAGAGTGA |
| Suggested primer 2: | AACGATACAAGCCCCAACAG |