| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.159358 |
| Chromosome: | chromosome 14 |
| Location: | 1686640 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619350 | (1 of 1) PTHR12169//PTHR12169:SF6 - ATPASE N2B // LACTATION ELEVATED PROTEIN 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCACCAACGCTGTGCGCACATCACCAGT |
| Internal bar code: | TACGGTCGGTAATCAACTTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 824 |
| LEAP-Seq percent confirming: | 99.8671 |
| LEAP-Seq n confirming: | 2254 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCAGGTCACATCCTCGTT |
| Suggested primer 2: | AGGACTTTGCTTCGGGTTTT |