| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.159365 |
| Chromosome: | chromosome 8 |
| Location: | 4349572 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g381650 | (1 of 1) PTHR12265//PTHR12265:SF0 - UNCHARACTERIZED // TRANSMEMBRANE PROTEIN 53 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCCGCACGCACACTCGCGACGCGCGCGG |
| Internal bar code: | GGATGTCGGCCGACACGCATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 192 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 53 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCCTTTCTCCCCCTCTTA |
| Suggested primer 2: | CTTGTAAGGAGCAAGGCTGG |