| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.159365 |
| Chromosome: | chromosome_17 |
| Location: | 2361825 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g714800 | ISG-C3,ISG6 | Hydroxyproline-rich cell wall protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GATAATGCGGCCCTGTCCCACGTGCCACAG |
| Internal bar code: | GGTCCCTTAATAGGCACTGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1138 |
| LEAP-Seq percent confirming: | 98.6906 |
| LEAP-Seq n confirming: | 2638 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCGGCTTATGTCATTCCC |
| Suggested primer 2: | GGCACTCCTGAGCTTATTGC |