| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.159370 |
| Chromosome: | chromosome 6 |
| Location: | 3102460 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g274950 | (1 of 1) K18953 - factor associated with neutral sphingomyelinase activation (NSMAF, FAN) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGACGTGCGCGTGCCCATTGCCAGGTGC |
| Internal bar code: | GCGGAAAGCCTGGAGCGTGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 335 |
| LEAP-Seq percent confirming: | 88.1142 |
| LEAP-Seq n confirming: | 7406 |
| LEAP-Seq n nonconfirming: | 999 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGTGTCCAAGATCCAGGG |
| Suggested primer 2: | GAGCCTCCACCAGAAGACTG |