| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.159384 |
| Chromosome: | chromosome_8 |
| Location: | 4703466 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g383950 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGCCACGGCGTCCAGCACGTAGGTCCCCTC |
| Internal bar code: | AGACTCGGTTGTGCTGTGAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 95.6098 |
| LEAP-Seq n confirming: | 196 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTCAAACTCACGTGCAAA |
| Suggested primer 2: | GCTTTTGTTGAATGGGTGCT |