| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.159414 |
| Chromosome: | chromosome 2 |
| Location: | 7093704 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g146950 | DBR1 | RNA lariat debranching enzyme; (1 of 1) K18328 - lariat debranching enzyme [EC:3.1.-.-] (DBR1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTTCGGACGGCGAGGGTGAAGGCGGGCC |
| Internal bar code: | TCCCCCGTGACAGCTCGGGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 988 |
| LEAP-Seq percent confirming: | 7.69231 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 108 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACACACACACACACCG |
| Suggested primer 2: | GTGCCTTCCCAACACAAGTT |