| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.159423 |
| Chromosome: | chromosome 12 |
| Location: | 9670212 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g554953 | ETF2,ETFB1 | Electron transfer flavoprotein beta subunit; (1 of 1) K03521 - electron transfer flavoprotein beta subunit (fixA, etfB) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCCCCCCTCGTGCCTGCCCGCTGTCCCT |
| Internal bar code: | CGTAGGCACAATGGTTGCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 239 |
| LEAP-Seq percent confirming: | 97.1246 |
| LEAP-Seq n confirming: | 304 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTCACACGACACAGGGAC |
| Suggested primer 2: | AGTCAGCCAAGGACACTGCT |